Tutorials

Phenix Tutorials

TEM-1 beta-blip (β-lactamase/β-lactamase inihibitor)

b-CA-mr (β-carbonic anhydrase)

ICAM-mr (intercellular adhesion molecule)

toxd-mr (α-Dendrotoxin)

CASP7-T0283-mr (ab-initio model)

HNP3-mr-rosetta (mr-rosetta)

Data

The coordinate (pdb), sequence (fasta) and reflection (mtz) files required to run the tutorials are distributed with Phaser.

They can also be obtained from:

Data for Molecular Replacement Examples and Tutorials

Data for Experimental Phasing Examples and Tutorials

Tutorials

The tutorials are suitable for individual use or class teaching.

• MR using CCP4i:TOXD
• MR using CCP4i:BETA/BLIP
• MR using keyword input
• Finding a search model:TOXD
• SAD using CCP4i
• Combined MR-SAD using CCP4i
• EP using Phenix

References

The crystal structure of a hetero-dimer of beta-lactamase (BETA) and beta-lactamase inhibitor protein (BLIP), both with molecular replacement models from crystal structures of the individual BETA and BLIP components. We thank Mike James and Natalie Strynadka for the diffraction data. Reference: Strynadka, N.C.J., Jensen, S.E., Alzari, P.M. & James. M.N.G. (1996) Nat. Struct. Biol. 3 290-297.

The crystal structure of insulin phased on intrinsic sulphurs. We thank Paul Adams for the diffraction data. Reference: Adams (2001) Acta Cryst D57. 990-995.